Aug 10 2021

A key pathway that might account for development of stress-induced TDP-43 protein aggregates in motor neurons of amyotrophic lateral sclerosis disease patients

Stephen Hawking, a world-famous theoretical physicist, was diagnosed with amyotrophic lateral sclerosis (ALS) at the age of 21. ALS is a rare motor neuron disease that usually affects individuals over the age of 60. Hawking survived for another 55 years, defying the odds. Hawking's illness, on the other hand, is an anomaly, since the majority of patients live just around 2-4 years after diagnosis. While the disease is rare, it has a major socioeconomic impact since people suffer motor degeneration and ultimately paralysis.

Although the cause of ALS is unclear, a team of Japanese scientists headed by Prof. Masahiro Fujii of Niigata University Graduate School of Medical and Dental Sciences and the Brain Research Institute is making significant progress in unraveling the underlying causative mechanisms. The accumulation of ubiquitinated TDP-43 (Ub-TDP-43) protein aggregates in motor neurons is a hallmark of ALS, and Prof. Fujii and colleagues have shown that a protein called optineurin (OPTN) inhibits the formation of Ub-TDP-43 aggregates in stress-induced stress granules (SGs), whereas the OPTN mutants derived from the ALS patients induce Ub-TDP-43 aggregates in SGs.

These SGs are generated in the cytoplasm of cells in response to a number of stressors, including heat shock and oxidation, and are subsequently eliminated spontaneously when the degree of stress diminishes. In their recent work, Prof. Fujii and fellow scientists, produced OPTN knockdown (OPTN-KD) cells and found an increase in the size of SGs, a delay in SG clearance and an accumulated Ub-TDP-43 in SGs after stress removal, which is characteristic of aberrant SGs. Additionally, they established a connection between this and an increase in the expression of TIA1, another protein implicated in the development of ALS.

This team of scientists demonstrated a direct link between OPTN-KD-induced increases in TIA1 protein and the formation of Ub-TDP-43-positive SGs in OPTN-KD cells (Figure 1).

Figure 1. Development of ALS. ALS-linked mutations of optineurin (OPTN) and depletion of OPTN delay clearance of SGs and increases ubiquitinated TDP-43 levels by increasing TIA1.

Due to the delayed clearance of these aberrant SGs, neurons accumulate Ub-TDP-43 aggregates, which show toxicity to neurons, culminating in the characteristic neuron degeneration seen in ALS patients.

"This discovery elucidates the process by which Ub-TDP-43 aggregates develop in the brains of ALS patients with optineurin or TIA1 mutations and explains how ubiquitinated proteins aggregate in neurodegenerative disorders such as ALS," Prof. Fujii explained in an interview.

Genetic variables, particularly mutations, are also important in ALS, particularly familial ALS. Previously, mutations in the OPTN and TIA1 genes were identified as causative factors in familial ALS with TDP-43 aggregation pathology. "Our findings indicate that ALS-associated OPTN loss-of-function mutations increase the quantity of Ub-TDP-43 in neurons via raising the expression of TIA1, thus enhancing Ub-TDP-43 aggregation," added Prof. Fujii.

To further understand the mechanisms by which OPTN suppresses TIA1 expression, the scientists looked at the NF-κB signaling pathway, since previous research has shown that OPTN inhibits NF-κB-mediated gene transcription. On the other side, the OPTN-KD increased the levels of TIA1 mRNA, whereas an NF-κB inhibitor had no effect, suggesting that this process is not NF-κB dependent (Figure 2). While it is a breakthrough that this study establishes a significant link between OPTN and aberrant SG formation, elevated TIA1 expression, ubiquitination, and TDP-43 aggregation, future research efforts should be directed toward understanding the mechanism by which OPTN regulates TIA1.

Figure 2. Key TIA1 suppression. Optineurin inhibits TIA1 transcription through an NF-κB-independent manner.

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The article "The optineurin/TIA1 pathway inhibits aberrant stress granule formation and reduces ubiquitinated TDP-43" was published on 23 July 2021 in iScience.
https://doi.org/10.1016/j.isci.2021.102733

In addition, these findings also build up on the following previously published articles:

"G3BP1 inhibits ubiquitinated protein aggregations induced by p62 and USP10"
https://doi.org/10.1038/s41598-019-46237-1

"USP10 is a critical factor for Tau-positive stress granule formation in neuronal cells"
https://doi.org/10.1038/s41598-019-47033-7

"USP10 Is a Driver of Ubiquitinated Protein Aggregation and Aggresome Formation to Inhibit Apoptosis"
https://doi.org/10.1016/j.isci.2018.11.006

Publication Details

Journal:?iScience
Title:?The optineurin/TIA1 pathway inhibits aberrant stress granule formation and reduces ubiquitinated TDP-43
Authors:?Taichi Kakihana, Masahiko Takahashi, Yoshinori Katsuragi, Shun-ichi Yamashita, Junya Sango, Tomotake Kanki, Osamu Onodera, and Masahiro Fujii
DOI: 10.1016/j.isci.2021.102733

篮球比分直播 release

The article was released in EurekAlert, the online publication of the American Association for the Advancement of Science.